Correction: Lesbon et al. Nucleocapsid (N) Gene Mutations of SARS-CoV-2 Can Affect Real-Time RT-PCR Diagnostic and Impact False-Negative Results. Viruses 2021, 13, 2474.

The authors hereby request the inclusion of two authors (Olivia Teixeira and Maria Cristina Nonato) in the recently published article in Viruses entitled "Nucleocapsid (N) gene mutations of SARS-CoV-2 can affect real-time RT-PCR diagnostic and impact false-negative results" [...].

Nucleocapsid (N) Gene Mutations of SARS-CoV-2 Can Affect Real-Time RT-PCR Diagnostic and Impact False-Negative Results.

The current COVID-19 pandemic demands massive testing by Real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction), which is considered the gold standard diagnostic test for the detection of the SARS-CoV-2 virus. However, the virus continues to evolve with mutations that lead to phenotypic alterations as higher transmissibility, pathogenicity or vaccine evasion. Another big issue are mutations in the annealing sites of primers and probes of RT-PCR diagnostic kits leading to false-negative results. Therefore, here we identify mutations in the N (Nucleocapsid) gene that affects the use of the GeneFinder COVID-19 Plus RealAmp Kit. We sequenced SARS-CoV-2 genomes from 17 positive samples with no N gene detection but with RDRP (RNA-dependent RNA polymerase) and E (Envelope) genes detection, and observed a set of three different mutations affecting the N detection: a deletion of 18 nucleotides (Del28877-28894), a substitution of GGG to AAC (28881-28883) and a frameshift mutation caused by deletion (Del28877-28878). The last one cause a deletion of six AAs (amino acids) located in the central intrinsic disorder region at protein level. We also found this mutation in 99 of the 14,346 sequenced samples by the Sao Paulo state Network for Pandemic Alert of Emerging SARS-CoV-2 variants, demonstrating the circulation of the mutation in Sao Paulo, Brazil. Continuous monitoring and characterization of mutations affecting the annealing sites of primers and probes by genomic surveillance programs are necessary to maintain the effectiveness of the diagnosis of COVID-19.

COVID-19 in the Perioperative Period of Cardiovascular Surgery: the Brazilian Experience.

INTRODUCTION: We investigated the clinical course and outcomes of patients submitted to cardiovascular surgery in Brazil and who had developed symptoms/signs of coronavirus disease 2019 (COVID-19) in the perioperative period. METHODS: A retrospective multicenter study including 104 patients who were allocated in three groups according to time of positive real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2): group 1, patients who underwent cardiac surgery > 10 days after positive RT-PCR; group 2, patients with a positive RT-PCR within 10 days before or after surgery; group 3, patients who presented positive RT-PCR > 10 days after surgery. The primary outcome was mortality and secondary outcomes were postoperative complications, intensive care unit (ICU) length of stay, and postoperative days of hospitalization. RESULTS: The three groups were similar with respect to age, the European System of Cardiac Operative Risk Evaluation score, and comorbidities, except hypertension. Postoperative complications and death were significantly higher in groups 2 and 3 than in group 1, and no significant difference between groups 2 and 3 was seen. Group 2 showed a high prevalence of surgery performed as an urgent procedure. Although no significant differences were observed in ICU length of stay, total postoperative hospitalization time was significantly higher in group 3 than in groups 1 and 2. CONCLUSION: COVID-19 affecting the postoperative period of patients who underwent cardiovascular surgery is associated with a higher rate of morbidity and mortality. Delaying procedures in RT-PCR-positive patients may help reduce risks of perioperative complications and death.

SARS-CoV-2 Delta variant of concern in Brazil - multiple introductions, communitary transmission, and early signs of local evolution (preprint)

The dissemination of the Delta VOC in Brazil is still unclear, despite the frequent reports of isolated cases from different Brazilian states. In this report we characterize the dissemination of the Delta VOC in Brazil and where the introductions of this lineage fall within the global Delta phylogeny. We also examined the mutational profile of the largest clade within the Brazilian Delta VOCs, with a focus on samples which were obtained in the State of Sao Paulo, and especially in the city of Sao Paulo, the largest metropolis of South America, and a national and international transportation hub.

Young favela dwellers and their relations with the city in times of pandemic [Jovens da Favela e suas relações com a cidade em tempos de pandemia]

An undergraduate student in the Arts course at a public university, the State University of Rio de Janeiro (UERJ), militant, non-binary transsexual and African Brazilian, Matheusa produced, in the engraving discipline, the zine "Rio de Janeiro is still beautiful and oppressive", where these verses come from. Keywords: Occupational science;Social occupational therapy;Anti-racism;Pandemic;Favela;Youth [Ciencia Ocupacional;Terapia ocupacional social;Anti-racismo;Pandemia;Juventude] EN Occupational science Social occupational therapy Anti-racism Pandemic Favela Youth [Ciencia Ocupacional Terapia ocupacional social Anti-racismo Pandemia Juventude] 449 456 8 08/09/21 20210801 NES 210801 I A body in the city i I A large exclusive city i I Phobic, racist, separatist, elitist city i I But Rio de Janeiro is still beautiful, isn't it? Available at https://elle.abril.com.br/cultura/o-legado-de-matheusa-passareli-e-sua-historia/ 2 The COVID-19 Unified Panel of the Favelas is a collective initiative of different social movements in the favelas of the state of Rio de Janeiro supported by the Institute of Communication and Information in Health (Icict), Health Information Laboratory (LIS), and Oswaldo Cruz Foundation (FIOCRUZ). COVID-19 e desigualdade: a distribuição dos fatores de risco no Brasil [COVID-19 and inequalities: Risk factors in Brazil].

Agentes Comunitários de Saúde frente à COVID-19: Vivências junto aos profissionais de enfermagem

Objetivo: Descrever a experiência vivenciada por enfermeiras em conexão com Agentes comunitários de saúde para enfrentamento local da pandemia COVID-19. Método: Relato de experiência desenvolvido a partir de ações de enfermeiras que atuam na Estratégia Saúde da Família do município de Icó, Estado do Ceará, destacando o importante papel que os Agentes comunitários de saúde desenvolvem junto à população nesse cenário. Resultados: A partir da experiência vivenciada, pode-se observar o importante papel que os Agentes Comunitários de Saúde têm apresentando frente à pandemia da COVD-19, mesmo apresentando medo e insegurança. Evidenciou-se ainda durante esse período que seu papel de educador em saúde é imprescindível nas ações desenvolvidas dentro dos territórios. Além disso, esses profissionais têm sido protagonistas importantes nas atividades realizadas dentro das Estratégias de Saúde da Família, fortalecendo assim o trabalho da equipe bem como o controle e combate da infecção pelo novo Coronavírus. Conclusões: Contar com o trabalho desses profissionais nos territórios tem sido estratégico e representa um diferencial para o cuidado, por auxiliarem no controle da disseminação e no monitoramento de grupos de risco, bem como das pessoas infectadas pela doença. (AU) Objective: To describe the experience lived by nurses in connection with community health workers to face the pandemic COVID-19 locally. Method: Experience report developed from the actions of nurses working in the Family Health Strategy in the municipality of Icó, State of Ceará, highlighting the important role that community health agents play with the population in this scenario. Results: Based on the experience, it is possible to observe the important role that Community Health Agents have playing in the face of the COVID-19 pandemic, even with fear and insecurity. It was also evident during this period that his role as a health educator is essential in the actions developed within the territories. In addition, these professionals have been important protagonists in the activities carried out within the Family Health Strategies, thus strengthening the team's work as well as controlling and fighting infection by the new Coronavirus. Conclusions: Counting on the work of these professionals in the territories has been strategic and represents a differential for care, as they help in controlling the dissemination and monitoring at-risk groups, as well as people infected by the disease. (AU) Objetivo: Describir la experiencia vivida por las enfermeras en relación con los trabajadores de salud de la comunidad para enfrentar la pandemia COVID-19. Método: Informe de experiencia desarrollado a partir de las acciones de enfermeras que trabajan en la Estrategia de Salud de la Familia en el municipio de Icó, Estado de Ceará, destacando el importante papel que los trabajadores de salud comunitarios juegan con la población en este escenario. Resultados: En base a la experiencia, es posible observar el importante papel que los agentes de salud comunitarios tienen ante la pandemia da COVID-19, incluso con miedo e inseguridad. También fue evidente durante este período que su papel como educador de salud es esencial en las acciones desarrolladas dentro de los territorios. Además, estos profesionales han sido protagonistas importantes en las actividades llevadas a cabo dentro de las Estrategias de salud familiar, fortaleciendo así el trabajo del equipo y controlando y luchando contra la infección por el nuevo Coronavirus. Conclusiones: contar con el trabajo de estos profesionales en los territorios ha sido estratégico y representa un diferencial para la atención, ya que ayudan a controlar la difusión y el monitoreo de los grupos en riesgo, así como a las personas infectadas por la enfermedad. (AU)